Journal: European Journal of Dentistry

Article Title: The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

doi: 10.1055/s-0043-1761460

Figure Lengend Snippet: The SHED-IL10 ( A ) and SHED-LL37 ( B ) concentration on the basic condition.

Article Snippet: The antibody used was human IL-10 (human IL-10, BT Laboratory, Shanghai, China) and human LL37 (human LL317, IL-10, BT Laboratory, Shanghai, China).

Techniques: Concentration Assay

Journal: European Journal of Dentistry

Article Title: The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

doi: 10.1055/s-0043-1761460

Figure Lengend Snippet: The SHED-IL10 ( A – C ) and SHED-LL37 ( E – F ) concentrations by two different epigallocatechin gallate (EGCG) concentrations and α-mangosteen. *** p < 0.001; **** p < 0.0001.

Article Snippet: The antibody used was human IL-10 (human IL-10, BT Laboratory, Shanghai, China) and human LL37 (human LL317, IL-10, BT Laboratory, Shanghai, China).

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Antimicrobial peptides against polymyxin-resistant Klebsiella pneumoniae : a patent review

doi: 10.1007/s11274-023-03530-6

Figure Lengend Snippet: Final selection of antimicrobial peptide patents that test against polymyxin-resistant K. pneumoniae

Article Snippet: US2020323950A1/ Abandoned , Antibacterial peptides and combinations for co-therapy (Kraus and Otvos ) , US/2020 , ARREVUS INC [US] , K. pneumoniae (polymyxin-resistant) , A61K38/12; A61K38/16; A61K45/06; A61P31/04.

Techniques: Selection, Mutagenesis

Journal: PLoS ONE

Article Title: An FPGA Implementation to Detect Selective Cationic Antibacterial Peptides

doi: 10.1371/journal.pone.0021399

Figure Lengend Snippet: Two physicochemical properties (Mean Charge, MC, Mean Hydrophobicity, MH) known to differentiate structured from non-structured proteins were applied to antibacterial peptides. The figure shows the paired values of a set of structured antibacterial peptides (empty squares) and unstructured antibacterial peptides (filled circles). See supplementary for further information about the peptide sequences used for this study.

Article Snippet: One example is the identification of selective cationic antibacterial peptides (SCAPs), which may be used in the treatment of different diseases.

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Antimicrobial peptides against polymyxin-resistant Klebsiella pneumoniae : a patent review

doi: 10.1007/s11274-023-03530-6

Figure Lengend Snippet: Final selection of antimicrobial peptide patents that test against polymyxin-resistant K. pneumoniae

Article Snippet: WO2020014642A2 WO2020014642A3/ Application filed , Antibacterial peptide monomers and combinations for co-therapy (Kraus and Otvos ) , WO/2020 , ARREVUS INC [US] , K. pneumoniae (polymyxin-resistant) , A61K39/02; A61K31/40; A61K31/56; A61K31/58; A61K31/675; A61K38/04; A61K38/10.

Techniques: Selection, Mutagenesis

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: Physicochemical properties of YI12 and FK13. # The isoelectric points (pI) of peptides were predicted by ExPASy ( https://web.expasy.org/protparam/ ), accessed on 20 July 2024.

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Sequencing

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: Characterization of YI12 and FK13 peptides. ( A ) Chemical structures of two AMPs. ( B ) Helical wheel projections of YI12 and FK13. Hydrophilic residues—circle shape, hydrophobic residues—diamond shape, potentially positively charged residues—pentagonal shape, potentially negatively charged residues—triangle shape (none). Hydrophobicity is color-coded: the most hydrophobic residues are green, and the amount of green decreases with hydrophobicity. Hydrophilic residues are coded in red, and the amount of red decreases with hydrophilicity. The residues that may be charged are light blue.

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques:

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: Antibacterial spectrum of YI12 and FK13 (MIC, μg/mL). MRSA, methicillin-resistant Staphylococcus aureus . G + , Gram-positive bacteria; G − , Gram-negative bacteria.

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Bacteria

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: The growth patterns and time-killing curves of the two MDR bacteria exposed to YI12 and FK13, respectively. ( A – D ) Growth patterns of MRSA T144 and E. coli B2 under YI12 and FK13. ( E , F ) Time-dependent killing curves of MRSA T144 and E. coli B2 by FK13. Bacteria isolates were grown to stationary phase and mixed with 0-, 1-, 5-, and 10-fold MIC of FK13. Data are representative of three independent experiments and shown as mean ± SEM. PBS, phosphate-buffered saline.

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Bacteria, Saline

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: CD spectrum of YI12 and FK13 under different solutions. Use PBS (10 mM, pH = 7.4), LPS (50 µM), SDS (50 mM), and 50% TFEA. Values are averages of three scans of samples with a peptide concentration of 100 μg/mL.

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Concentration Assay

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: Hemolytic activity evaluation of YI12 and FK13 toward mammalian RBCs. Sterilized PBS served as a negative control, and ddH2O served as a positive control.

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Activity Assay, Negative Control, Positive Control

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: Stability evaluation of antimicrobial peptides YI12 and FK13 against MRSA T144 and E. coli B2 (MIC, μg/mL).

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Control

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: YI12 and FK13 disrupt bacterial membrane permeability. ( A , D ) Outer membrane permeability of E. coli B2 after exposure to YI12 and FK13, respectively, determined using a fluorescent probe N -phenyl-1-naphthylamine (NPN, excitation wavelength 350 nm, emission wavelength 420 nm); ( B , C , E , F ) YI12 and FK13 disrupt bacterial membrane permeability. Detections were performed with the fluorescent probe propidium iodide (PI, excitation 535 nm, emission 615 nm). Statistical analysis was conducted through nonparametric one-way analysis (ns, not significant; ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Membrane, Permeability

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: Changes in membrane potential of E. coli B2 after exposure to YI12 ( A ) and FK13 ( C ), and MRSA T144 after exposure to YI12 ( B ) and FK13 ( D ). MRSA T144 and E. coli B2 membrane potential was determined by monitoring the fluorescence intensity of 3,3′-dipropylthiodicarbocyanine iodine (DiSC 3 (5)). Excitation wavelength 622 nm, emission wavelength 670 nm.

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: Membrane, Fluorescence

Journal: Pathogens

Article Title: Evaluation of the Antibacterial Potential of Two Short Linear Peptides YI12 and FK13 against Multidrug-Resistant Bacteria

doi: 10.3390/pathogens13090797

Figure Lengend Snippet: YI12 and FK13 trigger the generation of ROS in E. coli B2 and MRSA T144. Generation of ROS in E. coli B2 ( A , D ) and MRSA T144 ( B , E ), determined by 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA, excitation wavelength 488 nm, emission wavelength 525 nm). The addition of the ROS scavenger NAC significantly inhibited the effects of YI12 and FK13 on MRSA T144 and E. coli B2 ( C , F ). Statistical analysis was conducted through nonparametric one-way analysis (ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The antibacterial peptides YI12 and FK13 used in the experiment came from GL Biochem (Shanghai, China) and were both synthesized by peptide solid-phase synthesis (SPPS).

Techniques: